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Vazyme - 5min Universal Ligation Mix

SKU: C311-01

Description

5min Universal Ligation Mix is a ready-to-use 2 × premix solution, containing T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. This kit is suitable for sticky end ligation, blunt-end ligation and TA ligation. Buffer with optimized ligation enhancer makes the reaction more efficient and convenient. Ligation can be completed in 5 min at 25°C. The product can be used directly to transform many chemically competent cells.

Research Area

,Cloning/Mutagenesis, Traditional/TA Cloning

Images & Validation

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Application Notes
Compatible: Compatible with sticky ends, blunt ends, TA , linker or adapter ligation Fast: Ligation can be completed quickly in 5 minutes at 25°C Convenient: Reaction mixture can be used directly for bacterial transformation

Key Properties

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Storage & Handling

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StorageStore at -30 ~ -15°C and transport at ≤0°C.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

Available Sizes

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50 rxn
$ 300.00
DispatchUsually dispatched within 5-10 working days
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