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Rat Vascular Endothelial Cadherin (VE-Cadherin) ELISA Kit

SKU: orb2667529
Free Kit Promotion

Description

Rat Vascular Endothelial Cadherin (VE-Cadherin) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Application Notes
standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample Typesserum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range3.13-200 ng/mL
Sensitivity1.21 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5

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Protocol Information

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48 T
$ 300.00
96 T
$ 420.00
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