Rat Prostaglandin Endoperoxide Synthase 2 (PTGS2/COX-2) ELISA Kit

SKU: orb776545

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2(PTGS2).

Research Area

Cancer Biology, Gastroenterology & Hepatology, Infectious Disease & Virology, Metabolism Research, Protein Biochemistry, Stem Cell & Developmental Biology

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.065 ng/mL
Concentration	10 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

PGG/HS ELISA Kit, PGHS2 ELISA Kit, PHS2 ELISA Kit, HCox2 ELISA Kit, COX2 ELISA Kit, Cyclooxygenase 2 ELISA Kit, Prostaglandin G/H Synthase And Cyclooxygenase ELISA Kit, Prostaglandin H2 synthase 2 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P35355

UniProt Details

−

No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Rat
Dynamic Range:	125-8000pg/mL
Sensitivity:	75.00 pg/mL

Reactivity:	Rat
Dynamic Range:	125-8000pg/ml
Sensitivity:	75pg/ml