Rat Follistatin (FST) ELISA Kit

SKU: orb779134

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Follistatin(FS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Follistatin(FS).

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FST protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FST in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	1.57-100 ng/mL
Sensitivity	0.53 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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FST ELISA Kit, Activin-binding protein ELISA Kit

Quality Guarantee

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Quick Database Links

UniProt

P21674

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

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