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Description
Research Area
Inflammatory Mediators, Inflammation, Interleukins, Cell Biology, Kinases, TLR Signaling Pathway
Images & Validation
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Item 1 of 7
| Tested Applications | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /Test, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Porcine, Rabbit |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 RG(p-T)LA |
| Target | Irak1 |
| Molecular Weight | 78 kDa |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−IRAK(phospho T387); p-IRAK(phospho T387); IRAK1(phospho-Thr387); IRAK1(phospho-T387); IRAK1(p-Thr387); Il1rak; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 2; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; IRAK1; IRAK1_HUMAN; IRAK2; IRAK2; mPLK; mPLK; Pelle; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk.
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Quick Database Links
Gene Symbol
Irak1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Summers, Lauren et al. Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury Mol Cell Neurosci, 57, 93-103 (2013)
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