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NFkB (phospho-50) ELISA Kit

SKU: orb343759

Description

NFkB (phospho-50) ELISA Kit

Research Area

Product Categories/Products/ELISA Kits,Detection Kit Type: ELISA Kit

Images & Validation

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Tested ApplicationsELISA
Application Notes
Rockland's NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).

Key Properties

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ReactivityHuman
Concentration1 Kit
ConjugationUnconjugated

Storage & Handling

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StorageSee kit insert for complete instructions.
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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NF-kB Transcription Factor Kit, p50 kit, EIA kit, NF kappaB ELISA Assay
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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1 kit
$ 1,260.00
DispatchUsually dispatched within 5-10 working days
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Distributors

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