Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

NF-kB (phospho-65) ELISA Kit

SKU: orb343760

Description

NF-kB (phospho-65) ELISA Kit

Research Area

Product Categories/Products/ELISA Kits,Detection Kit Type: ELISA Kit

Images & Validation

−
Tested ApplicationsELISA
Application Notes
Rockland's NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).

Key Properties

−
ReactivityHuman
ConjugationUnconjugated

Storage & Handling

−
StorageSee kit insert for complete instructions.
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

−
NF-kB Transcription Factor Kit, p65 kit, EIA kit, NF-kB p65 Transcription Factor Assay
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
Find More Protocols

Available Sizes

Select a size below

1 kit
$ 1,260.00
DispatchUsually dispatched within 5-10 working days
Bulk Enquiry
Need technical support? Find your Distributor Return and Tax Policy

Email

[email protected]

UK Office

+44 (0) 1223 859-353

US Office

+1 (415) 906-5211

CN Office

+86 (27)-87663101

Distributors

biorbyt.com/distributor