NCAM / CD56 Antibody

SKU: orb385892

Description

CD56/NCAM is a cell adhesion protein which is a member of the immunoglobulin superfamily. It is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. CD56 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. [UniProt]

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Reactivity	Human
Application Notes	Titering of the CD56 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CDLA56
Immunogen	Recombinant human protein was used as the immunogen for the CD56 antibody.
Purity	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Store the CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Hazard Information	This CD56 antibody is available for research use only.
Disclaimer	For research use only

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Quick Database Links

UniProt

P13591

UniProt Details

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No UniProt data available

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin