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Description
Research Area
Product Categories/Products/Antibodies,PSC Type: Protein
Images & Validation
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Item 1 of 1
| Tested Applications | FC, SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Source | Mouse |
|---|---|
| Biological Origin | Mouse |
| Isotype | IgM |
| Conjugation | Unconjugated |
| Purity | Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE. |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. Mouse IgM Kappa (κ) isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide. 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
| Concentration | 1.0mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mouse immunoglobulin M kappa, Mouse IgM isotype control, mouse IgM kappa
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
FC
Flow Cytometry
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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