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Klenow Fragment

SKU: orb94056

Description

Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.

Images & Validation

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Application Notes
Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.

Key Properties

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PurityThe enzyme is greater than 98% pure as indicated by SDS-

Storage & Handling

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Storagestore at -20 °C
Form/Appearanceliquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)
Concentration5 units/µl
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Available Sizes

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5 x 300 units
$ 360.00
DispatchUsually dispatched within 5-10 working days
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