IL-33 Antibody

SKU: orb1272607

This product can be custom made

Description

IL-33 Antibody

Research Area

,Chemokines & Cytokines

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, IHC, NeA, WB
Reactivity	Human
Application Notes	ELISA:Sandwich:To detect hIL-33 by sandwich ELISA (using 100μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33. Western Blot:To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required.Immunohistochemistry:This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Immunogen	E.coli derived Recombinant Human IL-33
Target	IL33
Purification	IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, RP11-575C20.2, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

IL33

UniProt

O95760

RefSeq:O95760

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	O95760

Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Find More Protocols

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Rat
Reactivity:	Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Rat
Reactivity:	Human, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC-P, WB
Predicted Reactivity:	Rat
Reactivity:	Human, Mouse
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Human
Dynamic Range:	7.82-500 pg/mL
Sensitivity:	3 pg/mL

Applications:	ELISA, IHC-P, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

IL-33 Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Quality Guarantee

Quick Database Links

UniProt Details

NCBI Reference Sequences

Protocol Information

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