IL-1B Antibody (biotin)

SKU: orb1272466

This product can be custom made

Description

Research Area

,Immunology,Chemokines & Cytokines,Inflammasomes

Images & Validation

−

Item 1 of 3

Tested Applications	ELISA, WB
Reactivity	Rat
Application Notes	ELISA:Sandwich:To detect Rat IL-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-1β (XP-5180) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1β.Western Blot:To detect rat IL-1-β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-1-β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

−

Antibody Type	Primary Antibody
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat IL-1-β (rat Interleukin-1-β).
Target	Il1b
Purification	Anti-rat IL-1-β specific antibody was purified by affinity chromatography and then biotinylated.
Conjugation	Unconjugated

Storage & Handling

−

Storage	IL-1B antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Interleukin-1 betaIL-1 beta

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

Il1b

UniProt

Q63264

RefSeq:Q63264

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	Q63264

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Mouse
Dynamic Range:	15.63-1000 pg/mL
Sensitivity:	6.7 pg/mL

Reactivity:	Goat
Dynamic Range:	15.63-1000 pg/mL
Sensitivity:	4.83 pg/mL

Applications:	DOT, ELISA, IHC, WB
Reactivity:	Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Applications:	ELISA, WB
Reactivity:	Mouse
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Equine, Rabbit
Reactivity:	Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

IL-1B Antibody (biotin)

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Similar Products

Mouse Interleukin 1 Beta (IL1b) Microsample ELISA Kit [orb1736445]

Goat Interleukin 1 Beta (IL1b) ELISA Kit [orb1817387]

IL-1 Beta Antibody Biotin Conjugated [orb345233]