Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit

SKU: orb1946818

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Description

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human VIPR1. During the reaction, Human VIPR1 in the sample or standard competes with a fixed amount of Human VIPR1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human VIPR1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human VIPR1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Competitive
Assay Time	1.5H
Range	0.16-10ng/mL
Sensitivity	0.1 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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VIP-R-1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R-2, PACAP-R2, VPAC1

Quality Guarantee

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Quick Database Links

Gene Symbol

VIPR1

UniProt

P32241

UniProt Details

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No UniProt data available

Protocol Information

Find More Protocols

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.054 ng/mL

Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.052 ng/mL

Reactivity:	Human
Dynamic Range:	0.156-10ng/mL
Sensitivity:	0.045ng/mL

Reactivity:	Human
Dynamic Range:	0.156-10ng/mL
Sensitivity:	0.045ng/mL

Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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