Human Vascular Endothelial Cadherin (VE-Cadherin) ELISA Kit

SKU: orb2668110

Description

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.21 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5

Quality Guarantee

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Quick Database Links

UniProt

P33151

UniProt Details

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No UniProt data available

Protocol Information

Find More Protocols

Reactivity:	Human
Dynamic Range:	31.25-2000pg/mL
Sensitivity:	13.7 pg/mL

Reactivity:	Human
Dynamic Range:	0.16-10 μg/mL
Sensitivity:	32.1 ng/mL

Reactivity:	Human
Dynamic Range:	312 pg/ml - 20,000 pg/ml
Sensitivity:	< 10 pg/ml

Reactivity:	Human
Dynamic Range:	0.156-10ng/ml
Sensitivity:	0.094ng/ml

Reactivity:	Human
Dynamic Range:	3.9 ng/mL-250 ng/mL
Sensitivity:	0.98 ng/mL

Human Vascular Endothelial Cadherin (VE-Cadherin) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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