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Human Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

SKU: orb777159
Free Kit Promotion

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 1(SFRP1).

Research Area

Signal Transduction

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SFRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SFRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SFRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SFRP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.059 ng/mL
Concentration10 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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FRP1 ELISA Kit, FRP ELISA Kit, FRP-1 ELISA Kit, FrzA ELISA Kit, SARP2 ELISA Kit, Secreted apoptosis-related protein 2 ELISA Kit

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Quality Guarantee

Quality Guarantee

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UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 300.00
96 T
$ 420.00
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