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Human S100 Calcium Binding Protein B (S100B) Microsample Fast ELISA Kit

SKU: orb1806586

Description

Human S100 Calcium Binding Protein B (S100B) Microsample Fast ELISA Kit

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Stem Cell & Developmental Biology

Images & Validation

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Application Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human S100B. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityHuman
Sample Typescerebrospinal fluid, blood, Urine, amniotic fluid;Sample volume:25μL
Assay TypeSandwich
Assay Time3H
Range31.25-2000pg/mL
Sensitivity18.75 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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S1-B, NEF, S1Beta
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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No UniProt data available

Protocol Information

Available Sizes

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48 T
$ 350.00
96 T
$ 460.00
DispatchUsually dispatched within 5-10 working days
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