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Human Prostaglandin F2 Alpha (PGF2a) ELISA Kit

SKU: orb2668220

Description

Human Prostaglandin F2 Alpha (PGF2a) ELISA Kit

Research Area

Cardiovascular Research, Metabolism Research

Images & Validation

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Application Notes
standard: 800 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample TypesSerum, plasma, saliva and other biological fluids.
Assay TypeCompetitive
Assay Time2.5h
Range12.5-800 pg/mL
Sensitivity3.65 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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8-isoprostane; 8-iso-PGF2α

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Protocol Information

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48 T
$ 320.00
96 T
$ 450.00
DispatchUsually dispatched within 5-10 working days
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