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Human Proinsulin (PI) ELISA Kit

SKU: orb779264
Free Kit Promotion

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Proinsulin(PI) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proinsulin(PI).

Research Area

Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeCompetitive
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity5.18 pg/mL
Concentration1000 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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INS ELISA Kit, Pro-Insulin ELISA Kit, Preproinsulin ELISA Kit

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Quality Guarantee

Quality Guarantee

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 320.00
96 T
$ 450.00
DispatchUsually dispatched within 5-10 working days
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