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Human NGF protein

SKU: orb392185

Description

Recombinant human NGF protein is produced by our mammalian expression system and the target gene encoding Ser122-Arg239 is expressed.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Epigenetics,Neuroscience,Product Categories/Proteins

Images & Validation

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Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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SourceHuman cells
TargetNGF
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR

Storage & Handling

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StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.0.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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NGFB; Beta-nerve growth factor; Beta-NGF

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 100.00
50 μg
$ 240.00
500 μg
$ 1,260.00
DispatchUsually dispatched within 5-10 working days
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