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Human Ischemia Modified Albumin (IMA) ELISA Kit

SKU: orb779226
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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ischemia Modified Albumin(IMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ischemia Modified Albumin(IMA).

Research Area

Cardiovascular Research, Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 30000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IMA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma and other biological fluids
Assay TypeCompetitive
Assay Time2.5h
Range468.75-30000 ng/mL
Sensitivity156.9 ng/mL
Concentration30000 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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IMA

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 320.00
96 T
$ 450.00
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