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Description
Research Area
Disease Biomarkers
Images & Validation
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Item 1 of 3
| Application Notes |
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Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-6 R alpha, His Tag on HIS1K Biosensor, can bind Human IL-6, premium grade with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Target | IL-6 R alpha |
| Reactivity | Human |
| Tag | C-6×His |
| Expression Region | Leu 20 - Pro 365 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 39.4 kDa |
| Purity | 95% |
| Protein Sequence | NP_000556.1 |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
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| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
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Recombinant Human IL-6 R alpha Protein, His Tag [orb1550904]
> 92% by SDS-PAGE.
Recombinant Human IL-6 R alpha Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Leu20-Asp358) of human IL-6 R alpha (Accession #NP_000556.1) fused with a 6��His Tag at the C-terminus.
50 μg, 100 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000556.1 |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Tanemura, Yutaro et al. Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system Biology (Basel), 4, 161-172 (2015)
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