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Human IL-35 Protein, Fc Tag

SKU: orb1184774
ActiveBiologically Active

Description

Human IL-35, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha) (Accession # Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.3 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Human IL-35 R, His Tag at 5 µg/mL (100 µL/well) can bind Human IL-35, Fc Tag with a linear range of 0.002-0.625 µg/mL (QC tested).
TargetIL-35
ReactivityHuman
TagC-Fc
Expression RegionArg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha)
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW73.3 kDa
Purity90%
Protein SequenceNP_005746.2 & NP_000873.2

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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100 μg
$ 750.00
1 mg
$ 4,090.00
DispatchUsually dispatched within 5-10 working days
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