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Human IL-2 R alpha Protein, His Tag

SKU: orb257613
ActiveBiologically Active

Description

Human IL-2 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).

Research Area

Disease Biomarkers

Images & Validation

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Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginHuman
Biological ActivityHuman IL-2 R alpha, His Tag captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
TargetIL-2 R alpha
ReactivityHuman
TagC-10×His
Expression RegionGlu 22 - Cys 213
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW23.6 kDa
Purity95%
Protein SequenceNP_000408

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
ProteinNP_000408

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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100 μg
$ 660.00
1 mg
$ 3,630.00
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