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Human IL-12B Protein, His Tag

SKU: orb257709
ActiveBiologically Active

Description

Human IL-12B, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginHuman
Biological ActivityLoaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
TargetIL-12B
ReactivityHuman
TagC-10×His
Expression RegionIle 23 - Ser 328
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW36.6 kDa
Purity95%
Protein SequenceAAH67499.1

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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20 μg
$ 420.00
50 μg
$ 770.00
1 mg
$ 4,830.00
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