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Human IGF-II Protein, Fc Tag

SKU: orb257566
ActiveBiologically Active

Description

Human IGF-II, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Human IGFBP-3, His Tag at 0.5 µg/mL (100 µL/well) can bind Human IGF-II, Fc Tag with a linear range of 1.2-39 ng/mL (QC tested).
TargetIGF-II
ReactivityHuman
TagN-Fc
Expression RegionAla 25 - Glu 91
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW34.4 kDa
Purity95%
Protein SequenceP01344-1

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 100 mM Glycine, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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    The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IGF2 is approximately 35-55 kDa due to glycosylation.

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    Human IGF-II Protein, Fc Tag, Ala 25 - Glu 91 (Accession # P01344-1) was expressed from HEK293 cells.

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP01344

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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100 μg
$ 500.00
1 mg
$ 2,560.00
DispatchUsually dispatched within 5-10 working days
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