Human Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit

SKU: orb775473

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1(HSP90b1).

Research Area

Immunology & Inflammation, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.133 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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HSP90-B1 ELISA Kit, ECGP ELISA Kit, GP96 ELISA Kit, GRP94 ELISA Kit, TRA1 ELISA Kit, Glycoprotein 96 ELISA Kit, Tumor Rejection Antigen(gp96)1 ELISA Kit, 94 kDa glucose-regulated protein ELISA Kit, Endoplasmin ELISA Kit

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Quick Database Links

UniProt

P14625

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

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Reactivity:	Human
Dynamic Range:	0.625 ng/mL-40 ng/mL
Sensitivity:	0.156 ng/mL

Reactivity:	Human
Dynamic Range:	7.813-500pg/ml
Sensitivity:	4.688pg/ml

Reactivity:	Human
Dynamic Range:	78.125-5000pg/ml
Sensitivity:	46.875pg/ml

Reactivity:	Human
Dynamic Range:	0.312-20ng/mL
Sensitivity:	0.126ng/mL

Human Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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