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Human GM-CSF R alpha Protein, His Tag

SKU: orb545734
ActiveBiologically Active

Description

Human GM-CSF R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1).

Images & Validation

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Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginHuman
Biological ActivityHuman GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, premium grade with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested).
TargetGM-CSF R alpha
ReactivityHuman
TagC-10×His
Expression RegionGlu 23 - Gly 320
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW36.4 kDa
Purity95%
Protein SequenceNP_006131.2

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Available Sizes

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100 μg
$ 660.00
1 mg
$ 3,860.00
DispatchUsually dispatched within 5-10 working days
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