Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit

SKU: orb780289

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor Like Protein 1(FGFRL1).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGFRL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGFRL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGFRL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGFRL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.052 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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FGFR5 ELISA Kit, FHFR ELISA Kit, FGF homologous factor receptor ELISA Kit, FGFR-like protein ELISA Kit, Fibroblast growth factor receptor 5 ELISA Kit

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Quick Database Links

UniProt

Q8N441

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Human
Dynamic Range:	15.6 pg/ml - 1,000 pg/ml
Sensitivity:	< 10 pg/ml

Reactivity:	Human
Dynamic Range:	0.156-10ng/mL
Sensitivity:	0.051ng/mL

Reactivity:	Human
Dynamic Range:	0.156-10ng/mL
Sensitivity:	0.051ng/mL

Reactivity:	Human
Dynamic Range:	0.156-10ng/mL
Sensitivity:	0.051ng/mL