Human Enkephalin (ENK) ELISA Kit

SKU: orb779104

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Enkephalin(ENK) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Enkephalin(ENK).

Research Area

Neuroscience, Signal Transduction

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 800 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ENK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	12.5-800 pg/mL
Sensitivity	3.48 pg/mL
Concentration	800 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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ENK

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Reactivity:	Human
Dynamic Range:	0.156-10ng/ml
Sensitivity:	0.094ng/ml

Reactivity:	Human
Dynamic Range:	0.05 ng/mL-10 ng/mL
Sensitivity:	0.025 ng/mL

Reactivity:	Human
Dynamic Range:	15.62-1000pg/mL
Sensitivity:	6.85pg/mL

Reactivity:	Human
Dynamic Range:	15.6-1000pg/mL
Sensitivity:	6.85pg/mL

Human Enkephalin (ENK) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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