Human CD3E & CD3G Heterodimer Protein, Fc Tag

SKU: orb762448

Description

Human CD3E & CD3G Heterodimer Protein, Fc Tag & Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).

Research Area

Disease Biomarkers

Images & Validation

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Application Notes

Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 38.3 kDa (CD3E) and 36.9 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 43-55 kDa under reducing (R) condition, and 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

Key Properties

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Expression System	HEK293
Biological Origin	Human
Target	CD3E & CD3G
Reactivity	Human
Tag	C-Fc \| C-Fc
Expression Region	Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated
MW	38.3 kDa (CD3E) & 36.9 (CD3G)
Purity	95%
Protein Sequence	NP_000724.1 (CD3E) & AAI13831 (CD3G)

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

CD3E & CD3G

RefSeq (Protein):NP_000724.1

RefSeq:AAI13831, CD3

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000724.1
RefSeq	AAI13831, CD3

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

View Guide