Human Apelin 12 (AP12) ELISA Kit

SKU: orb775499

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 12(AP12) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 12(AP12).

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 6000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	93.75-6000 pg/mL
Sensitivity	25.28 pg/mL
Concentration	6000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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APLN12 ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Reactivity:	Human
Dynamic Range:	31.25 pg/mL-2000 pg/mL
Sensitivity:	7.81 pg/mL

Reactivity:	Human
Dynamic Range:	31.25-2000pg/ml
Sensitivity:	18.75pg/ml

Human Apelin 12 (AP12) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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