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Horse C Reactive Protein (CRP) ELISA Kit

SKU: orb782756
Free Kit Promotion

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein(CRP).

Research Area

Cancer Biology, Cardiovascular Research, Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse CRP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityEquine
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range6.25-400 ng/mL
Sensitivity2.49 ng/mL
Concentration400 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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C-RP ELISA Kit, PTX1 ELISA Kit, Pentraxin-Related ELISA Kit, Pentraxin 1 ELISA Kit
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

48 T
$ 300.00
96 T
$ 420.00
DispatchUsually dispatched within 5-10 working days
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