Thrombospondin Antibody

SKU: orb167695

This product can be custom made

Description

Goat polyclonal antibody to THBS1.

Images & Validation

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Item 1 of 9

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	WB: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Thrombospondin
Protein Sequence	NRIPESGGDNSVFD
Molecular Weight	129
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti THBS1 antibody, anti thrombospondin 1 antibody, anti THBS antibody, anti TSP antibody, anti TSP1 antibody, anti thrombospondin-1p180 antibody

Quality Guarantee

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Quick Database Links

Gene Symbol

Thrombospondin

NCBI Gene ID

7057

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Available Sizes

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100 μg

$ 500.00

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Applications:	ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	FC, IF, IHC-Fr, IHC-P, IP, WB
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Human
Dynamic Range:	3.13-200 ng/mL
Sensitivity:	1.22 ng/mL

Thrombospondin Antibody

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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