Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

SHH Antibody

SKU: orb527297

Description

Goat polyclonal antibody to SHH

Images & Validation

−
Tested ApplicationsELISA, FC, IF, WB
Dilution RangeP-ELISA: 1: 32000
ReactivityHuman, Mouse
Predicted ReactivityCanine, Rat
Application Notes
WB: Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular . Notes: Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Key Properties

−
HostGoat
ClonalityPolyclonal
TargetSHH
Protein SequenceDSEALHPLGMAVK
Molecular Weight49.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

−
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

−
SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

Similar Products

−
  • Shh Rabbit Polyclonal Antibody [orb11368]

    IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Equine, Gallus, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • SHH Rabbit Polyclonal Antibody [orb579101]

    IHC,  WB

    Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat, Zebrafish

    Gallus, Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • SHH [orb97480]

    FC,  IHC,  WB

    Human, Monkey, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μl
  • Human Hedgehog Homolog, Sonic (SHH) ELISA Kit [orb776079]

    Human

    78.13-5000 pg/mL

    28 pg/mL

    48 T, 96 T
  • Mouse Hedgehog Homolog, Sonic (SHH) ELISA Kit [orb1817384]

    Mouse

    15.63-1000 pg/mL

    5.51 pg/mL

    48 T, 96 T
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

−
No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

Please add an antibody product to your cart first.

DispatchUsually dispatched within 5-10 working days
Bulk Enquiry