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P2X4/P2X4R Antibody

SKU: orb19982

Description

Goat polyclonal antibody to P2RX4

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience

Images & Validation

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Tested ApplicationsELISA, FC, WB
Dilution RangeELISA: 1:128000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 60-70kDa band observed in Human Kideney, Liver and Rat Kidney (calculated MW of 43.4kDa according to NP_002551.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17;279(38):39485-94.; PMID: 15262999). Recommended concentration: 1-3µg/ml.

Key Properties

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HostGoat
ClonalityPolyclonal
TargetP2X4 / P2X4R
Protein SequenceYREKKYKYVEDYEQ
Molecular Weight43.4; 45.3; 40.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti P2X4 antibody, anti P2X4R antibody, anti P2RX4 antibody, anti purinergic receptor P2X, ligand-gated ion channel, 4 antibody, anti ATP receptor antibody, anti ATP-gated cation channel protein antibody, anti P2X purinoceptor 4 antibody, anti P2X receptor, subunit 4 antibody, anti purinergic receptor P2X ligand-gated ion channel 4 antibody, anti purinergic receptor P2X4 antibody, anti purinoceptor P2X4 antibody

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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