ITK Antibody

SKU: orb137166

This product can be custom made

Description

Goat polyclonal to ITK

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75ug/ml
Reactivity	Human, Rat
Predicted Reactivity	Bovine
Application Notes	WB: Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	ITK
Protein Sequence	KERPEDRPAFSR
Molecular Weight	71.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti ITK antibody

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