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Goat anti-Human IgM (µ chain), F(ab)'2 fragment, HRP conjugated

SKU: orb699966

Description

Goat polyclonal antibody against Goat anti-Human IgM (μ chain) conjugated to HRP

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Secondary Antibodies,Immunoassays, Immunology

Images & Validation

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Dilution Range1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Application Notes
This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

Key Properties

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HostGoat
ClonalityPolyclonal
PurityImmunogen affinity purified IgG, F(ab)'2 fragment.
ConjugationHRP

Storage & Handling

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StorageStore lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/AppearanceLyophilized
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Protocol Information

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0.5 mg
$ 650.00
Free Secondary Antibody (20 ul)0/0

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