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FOXA2/HNF3B Antibody

SKU: orb18541

Description

Goat polyclonal antibody to FOXA2

Research Area

Developmental Biology, Epigenetics, Stem Cells

Images & Validation

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Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 4-8 μg/ml
ReactivityHuman, Rat
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 48.3kDa according to NP_068556.1and NP_710141.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3 μg/ml.

Key Properties

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HostGoat
ClonalityPolyclonal
TargetFOXA2 / HNF3B
Protein SequenceGVYSRPIMNSS
Molecular Weight48.9; 48.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti FOXA2 antibody, anti HNF3B antibody, anti forkhead box A2 antibody, anti TCF3B antibody, anti MGC19807 antibody, anti hepatic nuclear factor-3-beta antibody, anti hepatocyte nuclear factor 3, beta antibody

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Quality Guarantee

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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