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FOXA1/HNF3A Antibody

SKU: orb18549

Description

Goat polyclonal antibody to FOXA1

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Tested ApplicationsELISA, FC, IF, WB
Dilution RangeELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml, FACS: 10ug/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Porcine, Rat
Application Notes
WB: Approx 50kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1kDa according to Human NP_004487.2 and 48.9kDa according to Mouse NP_032285.2).These bands we. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Key Properties

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HostGoat
ClonalityPolyclonal
TargetFOXA1 / HNF3A
Protein SequenceGVYSRPVLNTS
Molecular Weight49.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti FOXA1 antibody, anti HNF3A antibody, anti forkhead box A1 antibody, anti TCF3A antibody, anti MGC33105 antibody, anti hepatocyte nuclear factor 3, alpha antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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