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Clusterin/APOJ Antibody

SKU: orb19080

Description

Goat polyclonal antibody to CLU

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

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Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35-37kDa band observed in human colon, kidney and prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

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HostGoat
ClonalityPolyclonal
TargetClusterin / APOJ
Protein SequenceEKALQEYRKKHREE
Molecular Weight57.8; 52.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti Clusterin antibody, anti CLU antibody, anti APOJ antibody, anti CLI antibody, anti KUB1 antibody, anti Apolipoprotein J antibody, anti Apo-J antibody

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
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Free Secondary Antibody (20 ul)0/0

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