FLICA 660 Caspase-1 Assay Kit

SKU: orb1409917

Description

This in vitro assay employs the far-red fluorescent inhibitor probe 660-YVAD-FMK to label active caspase-1 enzyme in living cells. Analyze samples using fluorescence microscopy, or flow cytometry.

Research Area

,Product Categories/ELISA Kits

Images & Validation

−

Application Notes

1. Prepare samples and controls, 2. Dilute 10X Binding Buffer 1:10 with diH2O., 3. Wash cells in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer., 4. Dilute Annexin V-FITC 1:10 in PBS., 5. Dilute Propidium Iodide (PI) 1:2 in PBS., 6. Add diluted Annexin V-FITC to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells)., 7. Add diluted PI to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells)., 8. Incubate ~10 minutes, protected from light., 9. Dilute samples by adding 250 µL 1X Binding Buffer to each., 10. Analyze with a flow cytometer. Annexin V-FITC optimally excites at 494 nm and emits at 519 nm (typically read in FL1). PI optimally excites at 536 nm and has a peak emission at 617 nm (typically read in FL3).

Key Properties

−

Target	Caspase 1
Sample Types	Cell culture

Storage & Handling

−

Storage	2-8°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

Caspase 1

Protocol Information