DNA Damage (8-OHdG) ELISA Kit

SKU: orb1822520

Description

Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

Research Area

Images & Validation

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Item 1 of 3

Application Notes

1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.

Key Properties

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Reactivity	All
Sample Types	Urine, Cell Lysates, Plasma, Sample matrices
Range	0.94 - 60 ng/mL
Sensitivity	0.59 ng/mL

Storage & Handling

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Storage	4°C and -20°C
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit

Quality Guarantee

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Quick Database Links

Gene Symbol

DNA Damage (8-OHdG)

Protocol Information

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Dynamic Range:	1.56-100 ng/mL
Sensitivity:	0.59 ng/mL