Diaminobezidine (DAB) Kit

SKU: orb2652870

Description

Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).

Images & Validation

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Application Notes

Mixing of reagent: 1. To one ml of Buffer Substrate (BS) in a test tube, add two drop of Chromogen (C) reagent mix well. 2. This ready-to-use reagent is good for several hours. The unused DAB solution can be discarded according to city, county, state, province or country’s regulations. Application: IHC, ICC 1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water. 2. Add few drops of ready to use DAB reagent on tissue slides for 5-10 minutes at RT. (Higher temperature 37°C can also be used for DAB chromogen, however the incubation time should be determined by the individual lab) 3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. 4. Add counterstain compatible with DAB, we use hematoxylin 30-60 seconds (cat. # AR-6521) or any counterstain. 5. Wash with tap water, buffer pH 7.4 or over, followed by rinsing with distilled/deionized water. 6. Mount slide with aqueous mounting medium or organic mounting medium, after dehydration as described in the protocol for mounting medium.

Key Properties

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Storage & Handling

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Storage	2-8 °C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Diaminobezidine (DAB) Kit

Description

Images & Validation

Key Properties

Storage & Handling

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