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Cortisol EIA Kit

SKU: orb1822515
FeaturedFeatured Product

Description

Quantitative colorimetric detection of cortisol

Research Area

,Cell Signaling | Cancer | Oxidative Stress | Neuroscience

Images & Validation

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Application Notes
The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Key Properties

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ReactivityAll
Sample TypesDried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media
Range100 - 3200 pg/ml
Sensitivity17.3 pg/ml

Storage & Handling

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Storage4°C
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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(11β)-11,17,21-trihydroxypregn-4-ene-3,20-dione EIA Kit,
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol
Cortisol

Protocol Information

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Available Sizes

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96 T
$ 510.00
DispatchUsually dispatched within 2-3 weeks
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Email

[email protected]

UK Office

+44 (0) 1223 859-353

US Office

+1 (415) 906-5211

CN Office

+86 (27)-87663101

Distributors

biorbyt.com/distributor