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Description
Research Area
Product Categories/Products/Antibodies,Immunogen Type: Native Protein
Images & Validation
−
Item 1 of 12
| Tested Applications | DOT, ELISA, IHC, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:50,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:1,000 - 1:10,000 |
| Reactivity | Bovine, Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Collagen Type IV from human and bovine placenta |
| Purity | Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.18 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
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Quick Database Links
UniProt
RefSeq (Protein):NP_001290039.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001290039.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
DOT
Dot Blot
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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