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Description
Research Area
,Primary Antibodies;Cancer;Cell Biology;Signal Transduction;T cell activation;Chemokine signaling;Inflammation mediated by chemokine and cytokine signaling;Interleukin signaling
Images & Validation
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Item 1 of 7
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:10~50, IF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB10940,RB17180 |
| Target | This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF. |
| Molecular Weight | 84437 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
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Phospho-B-Raf-S445 polyclonal antibody [orb642228]
WB
Human, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl
Quality Guarantee
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Quick Database Links
Gene Symbol
This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF.
UniProt
RefSeq (Protein):NP_004324.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004324.2 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days