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Mouse IL-6 Protein, His Tag

SKU: orb750344
ActiveBiologically Active

Description

Mouse IL-6, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
Biological OriginMouse
Biological ActivityImmobilized Mouse IL-6, His Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Mouse IL-6 R alpha, His, with a linear range of 20-156 ng/mL (QC tested).
TargetIL-6
ReactivityMouse
TagN-10×His
Expression RegionPhe 25 - Thr 211
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW23.6 kDa
Purity90%
Protein SequenceNP_112445.1

Storage & Handling

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Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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20 μg
$ 420.00
50 μg
$ 750.00
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